Nanoparticles Induce Breast cancer Cell-line Caspase-Mediated Cell Death through Dosage Increase of Reactive Oxygen Species

Abstract

This study was carried out to examine the mode of interaction of Doxorubicin Loaded Aragonite Calcium Carbonate Nanoparticles with MCF-7 cell lines, to understand the effect of these agents on cell organic molecules. Cockle shells, antibiotics combination, Phosphate buffered saline, Doxorubicin hydrochloride, intracellular Reactive Oxygen Species Assay Kit Superoxide dismutase, were all used in the study.  The results of the investigation indicate that treatment of MCF-7 cells with DOX-Ar-CC-NPs and DOX exhibited a dose-dependent effect on cell viability. The ability of DOX-NPs to induce apoptosis in MCF-7 cells was confirmed; indicting the high potency of Aragonite Calcium Carbonate Nanoparticles in drug delivery.